External Resources
 12259https://www.addgene.org/12259
            
             Addgene identifier (12259, reused)
 12260https://www.addgene.org/12260
            
             Addgene identifier (12260, reused)
 96808https://www.addgene.org/96808
            
             Addgene identifier (96808, reused)
 178032https://www.addgene.org/178032
            
             Addgene identifier (178032, reused)
 medrxiv....ll.pdfhttps://www.medrxiv.org/content/10.1101/2025.02.07.25321793v2.full.pdf
            
             Article fulltext
 EMD-52165https://www.ebi.ac.uk/pdbe/emdb/EMD-52165
            
             EMBL-EBI EMDB identifier (EMD-52165)
 EMD-52458https://www.ebi.ac.uk/pdbe/emdb/EMD-52458
            
             EMBL-EBI EMDB identifier (EMD-52458)
 EMPIAR-12518https://www.ebi.ac.uk/empiar/EMPIAR-12518
            
             EMBL-EBI EMPIAR identifier (EMPIAR-12518)
 9606https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=9606
            
             NCBI taxonomy (human, Homo sapiens)
 1247190https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=1247190
            
             NCBI taxonomy (yeast, Saccharomyces cerevisiae BY4741)
 0000-0003-2864-0854https://orcid.org/0000-0003-2864-0854
            
             ORCID identifier (Mohammed Al-Azzani)
 0000-0002-6004-9872https://orcid.org/0000-0002-6004-9872
            
             ORCID identifier (Susana R. Chaves)
 0000-0001-6407-1199https://orcid.org/0000-0001-6407-1199
            
             ORCID identifier (Claudia Trenkwalder)
 0000-0001-7788-7074https://orcid.org/0000-0001-7788-7074
            
             ORCID identifier (Janin Lautenschläger)
 0000-0002-2536-6581https://orcid.org/0000-0002-2536-6581
            
             ORCID identifier (Markus Zweckstetter)
 0000-0002-8366-7622https://orcid.org/0000-0002-8366-7622
            
             ORCID identifier (Ruben Fernandez-Busnadiego)
 0000-0001-8437-3645https://orcid.org/0000-0001-8437-3645
            
             ORCID identifier (Brit Mollenhauer)
 0000-0003-1679-1727https://orcid.org/0000-0003-1679-1727
            
             ORCID identifier (Tiago Fleming Outeiro)
 1171https://prod.mbexc.uni-goettingen.de/literature/publications/1171
            
             Published Data Registry entry (MBExC)
 9HXAhttps://www.rcsb.org/structure/9HXA
            
             RCSB PDB Identifier (DOI:10.2210/pdb9HXA/pdb)
 04b6nzv94https://ror.org/04b6nzv94
            
             ROR identifier (04b6nzv94, Brigham and Women's Hospital)
 043j0f473https://ror.org/043j0f473
            
             ROR identifier (043j0f473, German Center for Neurodegenerative Diseases)
 03vek6s52https://ror.org/03vek6s52
            
             ROR identifier (03vek6s52, Harvard University)
 00cfam450https://ror.org/00cfam450
            
             ROR identifier (00cfam450, Helmholtz Zentrum München)
 03av75f26https://ror.org/03av75f26
            
             ROR identifier (03av75f26, Max Planck Institute for Multidisciplinary Sciences)
 05xy1nn52https://ror.org/05xy1nn52
            
             ROR identifier (05xy1nn52, Multiscale Bioimaging)
 021ft0n22https://ror.org/021ft0n22
            
             ROR identifier (021ft0n22, University Medical Center Göttingen)
 013meh722https://ror.org/013meh722
            
             ROR identifier (013meh722, University of Cambridge)
 037wpkx04https://ror.org/037wpkx04
            
             ROR identifier (037wpkx04, University of Minho)
 CVCL_0030https://scicrunch.org/resolver/CVCL_0030
            
             SciCrunch identifier (RRID:CVCL_0030, HeLa)
 SCR_018392https://scicrunch.org/resolver/SCR_018392
            
             SciCrunch identifier (RRID:SCR_018392, crYOLO)
 SCR_002798https://scicrunch.org/resolver/SCR_002798
            
             SciCrunch identifier (RRID:SCR_002798, GraphPad Prism)
 SCR_014565https://scicrunch.org/resolver/SCR_014565
            
             SciCrunch identifier (RRID:SCR_014565, GROMACS)
 SCR_016387https://scicrunch.org/resolver/SCR_016387
            
             SciCrunch identifier (RRID:SCR_016387, Illumina NovaSeq 6000 Sequencing System)
 SCR_016137https://scicrunch.org/resolver/SCR_016137
            
             SciCrunch identifier (RRID:SCR_016137, Microsoft Excel)
 SCR_016499https://scicrunch.org/resolver/SCR_016499
            
             SciCrunch identifier (RRID:SCR_016499, MotionCor2)
 SCR_016274https://scicrunch.org/resolver/SCR_016274
            
             SciCrunch identifier (RRID:SCR_016274, RELION)
 SCR_000305https://scicrunch.org/resolver/SCR_000305
            
             SciCrunch identifier (RRID:SCR_000305, PyMOL)
 SCR_014228https://scicrunch.org/resolver/SCR_014228
            
             SciCrunch identifier (RRID:SCR_014228, Sparky)
 SCR_014227https://scicrunch.org/resolver/SCR_014227
            
             SciCrunch identifier (RRID:SCR_014227, TopSpin)
 SCR_021351https://scicrunch.org/resolver/SCR_021351
            
             SciCrunch identifier (RRID:SCR_021351, Zeiss Axio Observer)
 SCR_020922https://scicrunch.org/resolver/SCR_020922
            
             SciCrunch identifier (RRID:SCR_020922, Zeiss LSM 780 Confocal Laser Scanning Microscope)
 SCR_022263https://scicrunch.org/resolver/SCR_022263
            
             SciCrunch identifier (RRID:SCR_022263, Zeiss LSM 900 with Airyscan 2)
 SCR_013672https://scicrunch.org/resolver/SCR_013672
            
             SciCrunch identifier (RRID:SCR_013672, ZEN Digital Imaging for Light Microscopy)
 medrxiv....d=truehttps://www.medrxiv.org/content/medrxiv/early/2025/02/14/2025.02.07.25321793/DC1/embed/media-1.pdf?download=true
            
             Supplemental material
 protocol...lpk/v1https://www.protocols.io/view/single-particle-analysis-of-synuclein-fibrils-81wgbxozylpk/v1
            
             Supplemental material (protocol)
 10.5281/zenodo.14730688https://doi.org/10.5281/zenodo.14730688
            
             Zenodo identifier (10.5281/zenodo.14730688)