View primary antibody

Basic data

PIDumg-sfb1286-antibody-primary-6
EPIC PIDhttps://hdl.handle.net/11022/umg-sfb1286-antibody-primary-6
Research groupRG Rizzoli
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolSNAP25
Antibody Registry ID(s)AB_887790
NameSNAP 25
Alternative namesynaptosome-associated protein 25
Lab ID
Tag / Fluorophore
Raised inrabbit
Reacts withhuman, rat, mouse, hamster, chicken, goldfish
Clone
Isotype
Clonalitypolyclonal
Demaskingunknown
AntigenSynthetic peptide corresponding to AA 192 to 206 from human SNAP25
Crafted Bycompany
Company / ManufacturerSynaptic Systems
Catalog no.111 002
Lot no.
DescriptionWB: 1 : 10000 (AP staining) IP: yes ICC: 1 : 500 IHC: 1 : 200 IHC-P/FFPE: 1 : 500 ELISA: yes (see remarks)
LocalizationSNAP 25 (synaptosome-associated protein of 25 kDa) is a highly conserved protein anchored to thecytosolic face of membranes via palmitoyl side chains in the middle of the molecule. SNAP 25 is thetarget of Botulinum neurotoxin A and E which cleave off 9 and 26 amino acids, respectively, from theC-terminus. SNAP 25 is part of the exocytotic fusion complex (v-SNARE) of neurons where it assembles withsyntaxin 1 and synaptobrevin. It is abundantly localized on the neuronal plasmalemma and onrecycling vesicles including synaptic vesicles. It is also expressed in neuroendocrine cells. There aretwo splice-variants, SNAP 25A and 25B.
Storage instruction
Receipt date2019-01-01 00:00:00
Preparation date2019-03-04 00:00:00
Created byMin, Ad
Last modifiedMin, Ad

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Linked publications


Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission
Truckenbrodt S, Viplav A, Jähne S, Vogts A, Denker A, Wildhagen H, Fornasiero EF, Rizzoli SO
EMBO J 2018 : 10.15252/embj.201798044