View primary antibody

Basic data

PID	umg-sfb1286-antibody-primary-271
EPIC PID	https://hdl.handle.net/11022/umg-sfb1286-antibody-primary-271
Research group	RG Rizzoli
Quality (mean)	no score (0.00)
Sharing level	Public

Antibody data

Antigen symbol	TUBA4A
Antibody Registry ID(s)	AB_2210057
Name	Anti-alpha Tubulin antibody
Alternative name	Alpha-tubulin 1 antibody
Lab ID
Tag / Fluorophore
Raised in	rabbit
Reacts with	mouse, rat, human
Clone
Isotype	IgG
Clonality	polyclonal
Demasking	unknown
Antigen	Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human alpha Tubulin.
Crafted By	company
Company / Manufacturer	Abcam
Catalog no.	ab18251
Lot no.
Description	ICC/IF Use a concentration of 1 - 5 µg/ml. Flow Cyt Use 0.01µg for 106 cells. WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). Abcam recommends using milk as the blocking agent.
Localization	Cytoplasm > cytoskeleton.
Storage instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Receipt date	2025-05-09 00:00:00
Preparation date	2025-05-09 00:00:00
Created by	Min, Ad
Last modified	Min, Ad

External links

Antibodypedia	-
HGNC	http://www.genenames.org/cgi-bin/gene_symbol_report?match=TUBA4A
Wikipedia	http://en.wikipedia.org/wiki/TUBA4A
Uniprot	http://www.uniprot.org/uniprot/?query=TUBA4A
GeneCards	http://www.genecards.org/cgi-bin/carddisp.pl?gene=TUBA4A
Antibody Registry	http://antibodyregistry.org/search?q=AB_2210057

Applications

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Linked publications

One-step nanoscale expansion microscopy reveals individual protein shapes
Shaib AH, Chouaib AA, Chowdhury R, Altendorf J, Mihaylov D, Zhang C, Krah D, Imani V, Spencer RKW, Georgiev SV, Mougios N, Monga M, Reshetniak S, Mimoso T, Chen H, Fatehbasharzad P, Crzan D, Saal K, Alawieh MM, Alawar N, Eilts J, Kang J, Soleimani A, Müller M, Pape C, Alvarez L, Trenkwalder C, Mollenhauer B, Outeiro TF, Köster S, Preobraschenski J, Becherer U, Moser T, Boyden ES, Aricescu AR, Sauer M, Opazo F, Rizzoli SO
Nat Biotechnol 2024 : 10.1038/s41587-024-02431-9

Microtubules as a versatile reference standard for expansion microscopy
Chowdhury R, Mimoso T, Chouaib AA, Mougios N, Krah D, Opazo F, Köster S, Rizzoli SO, Shaib AH
Commun Biol 2025 : 10.1038/s42003-025-07967-3