View primary antibody

PID	umg-sfb1286-antibody-primary-260
EPIC PID	https://hdl.handle.net/11022/umg-sfb1286-antibody-primary-260
Research group	RG Outeiro
Quality (mean)	no score (0.00)
Sharing level	Public

Antigen symbol	GFAP
Antibody Registry ID(s)	AB_304558
Name	Anti-GFAP antibody
Alternative name	intermediate filament protein
Lab ID
Tag / Fluorophore
Raised in	chicken
Reacts with	mouse, rat
Clone
Isotype	IgY
Clonality	polyclonal
Demasking	unknown
Antigen	Recombinant full length protein corresponding to Human GFAP. Isotype 1 expressed in and purified from E. coli.
Crafted By	company
Company / Manufacturer	Abcam
Catalog no.	ab4674
Lot no.
Description	IHC (PFA fixed) Use at an assay dependent concentration. IHC-FrFl 1/1000 - 1/5000. Try this antibody at about between about 1:1,000 using fluorescent secondary antibodies or 1:5,000 using peroxidase or other enzyme linked methods. ICC Use a concentration of 1 mg/ml. IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. WB 1/1000 - 1/5000. Predicted molecular weight: 50 kDa. Expect to see a band at 55kDa and another at about 48kDa, apparently a breakdown product of the 55kDa band.
Localization
Storage instruction	Shipped at 4°C. Store at +4°C. Do Not Freeze.
Receipt date	2025-05-08 00:00:00
Preparation date	2025-05-08 00:00:00
Created by	Min, Ad
Last modified	Min, Ad

Antibodypedia	-
HGNC	http://www.genenames.org/cgi-bin/gene_symbol_report?match=GFAP
Wikipedia	http://en.wikipedia.org/wiki/GFAP
Uniprot	http://www.uniprot.org/uniprot/?query=GFAP
GeneCards	http://www.genecards.org/cgi-bin/carddisp.pl?gene=GFAP
Antibody Registry	http://antibodyregistry.org/search?q=AB_304558