View primary antibody

PID	umg-sfb1286-antibody-primary-259
EPIC PID	https://hdl.handle.net/11022/umg-sfb1286-antibody-primary-259
Research group	RG Outeiro
Quality (mean)	no score (0.00)
Sharing level	Public

Antigen symbol	SNCA
Antibody Registry ID(s)	AB_869973
Name	Recombinant Anti-Alpha-synuclein (phospho S129) antibody
Alternative name	Alpha synuclein antibody
Lab ID
Tag / Fluorophore
Raised in	rabbit
Reacts with	mouse, rat, human
Clone	EP1536Y
Isotype	IgG
Clonality	monoclonal
Demasking	unknown
Antigen	Synthetic peptide within Human Alpha-synuclein aa 100 to the C-terminus. The exact sequence is proprietary
Crafted By	company
Company / Manufacturer	Abcam
Catalog no.	ab51253
Lot no.
Description	IHC-FrFl Use at an assay dependent concentration. WB 1/1000 - 1/5000. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).Can be blocked with Alpha-synuclein (phospho S129) peptide (ab188826). Good results have been obtained by treating the membrane with 0.4% PFA for 30 min at room temperature before blocking it with 5% milk. Dot blot Use at an assay dependent concentration. ELISA Use at an assay dependent concentration. IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Localization	Cytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons.
Storage instruction	Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Receipt date	2025-05-08 00:00:00
Preparation date	2025-05-08 00:00:00
Created by	Min, Ad
Last modified	Min, Ad

Antibodypedia	-
HGNC	http://www.genenames.org/cgi-bin/gene_symbol_report?match=SNCA
Wikipedia	http://en.wikipedia.org/wiki/SNCA
Uniprot	http://www.uniprot.org/uniprot/?query=SNCA
GeneCards	http://www.genecards.org/cgi-bin/carddisp.pl?gene=SNCA
Antibody Registry	http://antibodyregistry.org/search?q=AB_869973