View primary antibody

Basic data

PIDumg-sfb1286-antibody-primary-230
EPIC PIDhttps://hdl.handle.net/11022/umg-sfb1286-antibody-primary-230
Research groupRG Rizzoli
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolSNAP29
Antibody Registry ID(s)AB_1142975
NameAnti-SNAP29 antibody
Alternative namesoluble 29 kDa NSF attachment protein
Lab ID
Tag / Fluorophore
Raised inrabbit
Reacts withmouse, rat
Clone
IsotypeIgG
Clonalitypolyclonal
Demaskingunknown
AntigenA synthetic peptide from part of rat SNAP29, conjugated to an immunogenic carrier protein
Crafted Bycompany
Company / ManufacturerAbcam
Catalog no.ab68824
Lot no.
DescriptionWB 1/300 - 1/3000. Predicted molecular weight: 29 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein. IHC-Fr 1/300 - 1/3000. ICC/IF 1/2000. IHC-P 1/300 - 1/3000 Cytoplasm. Membrane. Cell junction > synapse > synaptosome. Appears to be mostly membrane-bound, probably via interaction with syntaxins, but a significant portion is cytoplasmic. Anti-SNAP29 antibody (ab68824) is not available anymore
Localization
Storage instructionShipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Receipt date2021-08-05 00:00:00
Preparation date2021-08-05 00:00:00
Created byMin, Ad
Last modifiedMin, Ad

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Linked publications


A comparative analysis of the mobility of 45 proteins in the synaptic bouton
Reshetniak S, Ußling J, Perego E, Rammner B, Schikorski T, Fornasiero EF, Truckenbrodt S, Köster S, Rizzoli SO
EMBO J 2020 : 10.15252/embj.2020104596