View primary antibody

Basic data

PIDumg-sfb1286-antibody-primary-15
EPIC PIDhttps://hdl.handle.net/11022/umg-sfb1286-antibody-primary-15
Research groupRG Rizzoli
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolSLC32A1
Antibody Registry ID(s)AB_2189809
NameVGAT
Alternative namesolute carrier family 32 member 1
Lab ID
Tag / Fluorophore
Raised inrabbit
Reacts withhuman, rat, mouse
Clone
Isotype
Clonalitypolyclonal
Demaskingunknown
AntigenSynthetic peptide corresponding to AA 510 to 525 from rat VGAT
Crafted Bycompany
Company / ManufacturerSynaptic Systems
Catalog no.131 103CpH
Lot no.
DescriptionWB: N/A IP: N/A ICC: 1 : 100 up to 1 : 200 IHC: yes IHC-P/FFPE: not tested yet
Localization
Storage instruction50 μg specific antibody, lyophilized. Affinity purified with the immunogen,fluorescence-labeled with CypHer5E. Rabbit serum albumin was added forstabilization. For reconstitution add 50 μl H2O to get a 1mg/ml solution in PBS.Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or storealiquots at -80°C without additives.Reconstitute immediately upon receipt! Avoid bright light when working with theantibody to minimize photo bleeching of the fluorescent dye.
Receipt date2019-01-01 00:00:00
Preparation date2019-03-04 00:00:00
Created byMin, Ad
Last modifiedMin, Ad

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Linked publications


Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission
Truckenbrodt S, Viplav A, Jähne S, Vogts A, Denker A, Wildhagen H, Fornasiero EF, Rizzoli SO
EMBO J 2018 : 10.15252/embj.201798044